ACPs in Severe PAD/CLI by Direct Intramuscular Injection

Inclusion Criteria: * Subjects will have one or more clinical indications diagnostic of CLI such as: distal extremity pain at rest that requires the subject to use analgesics for \>2 weeks; or peripheral ischemic ulcer(s); or areas of gangrene ; or non-healing ischemic ulcers AND * Subjects will have one or more of the following hemodynamic indicators of severe peripheral arterial occlusive disease: * Ankle brachial index \< 0.45 * Toe brachial index \< 0.35 * TcPO2 / TcO2 of \< 40 mmHg. * The subject is a poor candidate for standard revascularization treatment options for peripheral arterial disease, based on inadequate bypass conduit, or unfavorable anatomy * Age 18 to 80 years * Male or non-pregnant, non-lactating female * Informed consent obtained and consent form signed Exclusion Criteria: * Patient having on angiography a meaningful supra popliteal occlusion that may relate to symptoms of CLI. * Subjects, who in the opinion of the investigator, have a vascular disease prognosis that indicates they would require a major amputation (at or above the ankle) within 4 weeks of start of treatment * Patient who received blood transfusions during the previous 4 weeks (to exclude the potential of non-autologous ACPs in the harvested blood). * Inability to communicate (that may interfere with the clinical evaluation of the patient) * Major operation during the preceding 3 months * Myocardial infarction or brain infarction or uncontrolled myocardial ischemia or persistent severe heart failure (EF\< 25 %) during the preceding 3 months * Significant valvular disease or after valve replacement during the preceding 3 months * After heart transplantation * Severe cardiomyopathy (EF \< 25 %) * Renal failure (creatinine \> 2 mg/dl ) * Hepatic failure * Anemia (lower than 11mg/dl.hemoglobin for female and lower than 12 mg/dl for male) * Abnormal coagulation tests \[platelets, PT (INR), PTT\] * Stroke within the preceding 3 years * Malignancy within the preceding 3 years * Concurrent chronic or acute infectious disease * Severe concurrent medical disease (e.g., septicemia, HIV-1,2/HBV/HCV infections, poorly controlled insulin-dependent diabetes mellitus ; HBA1c \>8 % and proliferative retinopathy , systemic lupus erythematosus, multiple sclerosis, amyotrophic lateral sclerosis) * Chronic immunomodulating or cytotoxic drugs treatment * Patients who have rectal temp. above 38.40C for 2 consecutive days * Patient unlikely to be available for follow-up